Nuclear deformation during breast cancer cell transmigration

The Significance of Nuclear Deformation for Cancer Cell Transmigration

In this paper, we present a microfluidic platform for the study of cancer cell transmigration and anti-cancer drug screening. The device consists of an array of microchannels with dimensions close to the capillaries on the cellular barriers, while a thin glass coverslip is used as the substrate to enable high resolution imaging of cell dynamics during cancer cell transmigration. We have investi...

Cell Fate Decisions During Breast Cancer Development

During the formation of breast cancer, many genes become altered as cells evolve progressively from normal to a pre-malignant to a malignant state of growth. How mutations in genes lead to specific subtypes of human breast cancer is only partially understood. Here we review how initial genetic or epigenetic alterations within mammary epithelial cells (MECs) can alter cell fate decisions and put...

FGFR1 cleavage and nuclear translocation regulates breast cancer cell behavior

FGF-10 and its receptors, FGFR1 and FGFR2, have been implicated in breast cancer susceptibility and progression, suggesting that fibroblast growth factor (FGF) signaling may be co-opted by breast cancer cells. We identify a novel pathway downstream of FGFR1 activation, whereby the receptor is cleaved and traffics to the nucleus, where it can regulate specific target genes. We confirm Granzyme B...

Polymorphonuclear Cell Transmigration

Color Based Segmentation of Nuclear Stained Breast Cancer Cell Images

We present an algorithm for segmenting cells in a microscopic image of immunohistologically stained slides from breast cancer based on color contents. The procedure for the approach consists of color categorization using neural network, noise removal and shape simplification using mathematical morphology, and cell size consideration. In order to obtain the more accurate segmentation, we further...